We provide a complete biodiversity assessment solution, including sampling design, training field technicians, DNA analysis, interpretation and report generation. This involves the analysis of bulk environmental samples such as water, soil, sediments and various types of sampled biomass, such as plankton trawls and benthic kicknets, to determine the composition of organisms from microbial groups to more complex organisms such as algae, plants, arthropods, nematodes and various higher vertebrates.
The approach is based on “DNA metabarcoding”, which employs information in marker genes such as ribosomal and mitochondrial genes that are amplified using CEGA’s optimized primers (synthetic DNA complimentary to conserved regions of target genes) and sequenced on high-throughput sequencers like the Illumina MiSeq and our in-house state-of-the-art Illumina NovaSeq 6000.
During the procedure, we sample small volumes of biological material, such as water or soil. Depending on the setting, water can be processed and filtered in field stations or on vessels. Small filter cartridges are then transferred back to our laboratory for further analysis.